Induction of Apoptosis with Moringa oleifera Fruits in HCT116 Human Colon Cancer Cells Via Intrinsic Pathway.
10.20307/nps.2017.23.4.227
- Author:
Tae Eun GUON
1
;
Ha Sook CHUNG
Author Information
1. College of Natural Sciences, Duksung Women's University, Seoul 01369, Korea. hasook@duksung.ac.kr
- Publication Type:Original Article
- Keywords:
Moringa oleifera fruits;
Cytotoxicity;
Intrinsic pathway
- MeSH:
Apoptosis*;
Blotting, Western;
Cell Size;
Colon*;
Colonic Neoplasms*;
Colorectal Neoplasms;
Cytotoxins;
Ethanol;
Flavonoids;
Fruit*;
Humans*;
Moringa oleifera*;
Moringa*;
Trees
- From:Natural Product Sciences
2017;23(4):227-234
- CountryRepublic of Korea
- Language:English
-
Abstract:
Moringa oleifera Lam (M. oleifera, Moringaceae) is a tree of the Moringaceae family that can reach a height of between 5 and 10 m. The current paper presents cytotoxic effect of M. oleifera fruits and its flavonoids 1 and 2. The viability of HCT116 human colon cancer cells were 38.5% reduced by 150 µg/mL of ethanolic extracts in a concentration-dependent manner; in addition, we observed the apoptotic features of cell shrinkage and decreased cell size. Bcl-2 family proteins were regulated as determined by Western blotting analysis, suggesting that M. oleifera fruits and their flavonoids 1 and 2 induced apoptosis through an intrinsic pathway. Based on our findings, 70% ethanolic extracts of M. oleifera fruits and flavonoids 1 and 2 might be useful as cytotoxic agents in colorectal cancer therapy.
