Variation of reactive oxygen species induced by arsenic trioxide in human hepatocellular carcinoma cell line
- VernacularTitle:三氧化二砷对肝癌细胞内活性氧水平的影响
- Author:
Junda LIU
;
Xiuyin YE
;
Xianbing CAI
- Publication Type:Journal Article
- Keywords:
Carcinoma,hepatocellular;
Arsenicals;
Reactive oxygen species
- From:
Chinese Journal of Primary Medicine and Pharmacy
2006;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of arsenic trioxide(As_2O_3) on reactive oxygen species(ROS) of human hepatocellular carcinoma cell(HCC) line.Methods The MTT method was used to evaluate the influence of As_2O_3 on proliferation of HCC.ROS was detected by flow cytometry.Results As_2O_3 could obviously decrease the proliferation rate of HCC cell line in dose-dependent and time-dependent manner.The analysis of flow cytometry showed As_2O_3 could obviously increase ROS in dose-dependent and time-dependent manner as well.Conclusion As_2O_3 can inhibit the proliferation of hepatic cell carcinoma SMMC-7721 cell line significantly,and the anti-tumours mechanism of arsenic trioxide may be that it could induce SMMC-7721 cell apoptosis by increasing ROS level.
