Effect of dexamethasone Angelica sinensis polysaccharide prodrug on trinitrobenzene sulfonic acid induced ulcerative colitis in rats
- VernacularTitle:地塞米松当归多糖前体药对三硝基苯磺酸诱导的大鼠溃疡性结肠炎的治疗作用
- Author:
Xinyou LIU
;
Siyuan ZHOU
;
Jianfeng CHENG
;
Zenghui TENG
;
Yuhua RAN
;
Runtao YANG
;
Xi YANG
;
Qibing MEI
- Publication Type:Journal Article
- Keywords:
ulcerative colitis;
trinitrobenzene sulfonic acid;
dexamethasone prodrug;
angelica sinensis polysaccharide
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2004;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To explore the therapeutic effect of dexamethasone Angelica sinensis polysaccharide prodrug(DEX-AP) on trinitrobenzene sulfonic acid(TNBS) induced ulcerative colitis(UC) in rats and its side effects.METHODS: The experimental UC rats were induced by clusis of the solution of TNBS in 45% alcoho1(50(mg?ml~(-1))).The UC rats were orally administrated with(0.25)(?mol?kg~(-1)?d~(-1)) DEX and(0.05),(0.25),(1.25)(?mol?kg~(-1)?d~(-1)) DEX-AP(calculated by carried DEX in DEX-AP) for 7 days,respectively.The rats were killed after the amount of peripheral blood lymphocyte was counted,then the spleen,thymus and colon were separated and weighted.After the ulcerative area of colon was calculated,the colonic myeloperoxidase(MPO) activity was determined and parts of colon were paraffin sectioned and examined under light microscope by HE stain.RESULTS: After the UC rats were administrated with different doses of DEX-AP for 7 days,the ulcerative area,the weight and the MPO activity of colon reduced significantly.The reduction of MPO activity was correlated to the dose of DEX-AP and the MPO activity with DEX-AP at the doses of(0.25),(1.25)(?mol?kg~(-1)?d~(-1)) reduced more significantly than that with DEX at the the dose of(0.25)(?mol?kg~(-1)?d~(-1)).The number of peripheral blood lymphocyte,spleen weight and thymus weight of UC rats reduced significantly at the dose of(0.25)(?mol?kg~(-1)?d~(-1)) DEX(P
