Inhibition of wound healing of lens epithelial monolayer wounds by an applied electric field
- VernacularTitle:电场暴露抑制晶状体上皮伤口的愈合
- Author:
Enpu WANG
;
Entong WANG
- Publication Type:Journal Article
- Keywords:
electric field;
lens epithelium;
cell movement;
wound healing
- From:
Ophthalmology in China
1993;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objectives To investigate the effects of electric field on the healing of lens epithelial cell wounds. Design Experimental study. Participants The cultured bovine lens epithelial cells and monolayers. Methods Small circle wounds were made by stabbing the cultured bovine lens epithelial monolayers with a glass needle and exposed to an applied electric field at a physiological strength of 200 mV/mm for 2 hours (n=37), and the wounds with no electric field exposure were used as control (n=29). With an image analyzer (Leica), the wound areas at 0, 1 and 2 hours after wounding were measured. Wounds were stained with F-actin fluorescence antibodies to observe the arrangement of microfilament cytoskeleton. Main Outcome Measures The wound areas and the arrangement of microfilament cytoskeleton of wounds. Results Compared to control wounds, the healing of wounds exposed to electric field was reduced, the mean area of field-exposed wounds at 2 hours after wounding (19106?m2?2167?m2) was significantly more than that of control wounds (8555?m2?1911?m2) (t=2.942, P=0.0045). The arrangement of microfilament cytoskeleton in control wounds was centripetally and a purse string-like arrangement of microfilament cytoskeleton was showed in field-exposed wounds. Conclusion Applied electric field at a physiological strength inhibits the healing of lens epithelial wounds.
