Therapeutic effects of tumor-selective replication-competent adenovirus carrying human endostatin gene on ovarian cancer:an in vivo study in mice
- VernacularTitle:携带人内皮抑素基因的肿瘤特异性增殖型腺病毒对卵巢癌的体内治疗作用
- Author:
Xiong CHEN
;
Jinyan SHA
- Publication Type:Journal Article
- Keywords:
endostatins;
adenoviridae;
gene therapy;
ovarian neoplasms
- From:
Academic Journal of Second Military Medical University
2000;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the inhibitory effect of tumor-selective replication-competent adenovirus-mediated human endostatin(CX-hE)on transplanted ovarian cancer(OV-90) in nude mice.Methods: BALB/c nude mice were inoculated subcutaneously with OV-90 cells to establish the animal model bearing human ovarian cancer.Eighteen nude mice bearing cancer were divided into 3 groups to receive intratumoral injection of PBS,CX-hE or Ad-hE,1/2 d,5 times.Then their livers were harvested for pathologic examination.Another 15 nude mice were divided into 3 groups to receive single intratumoral injection of CX-hE,Ad-hE or ONYX-015.Venous blood was collected on day 1,3 and 7 after injection for hEndo measurement by ELISA.The tumors were harvested for pathologic examination and immunohistochemical staining.Results: The tumors grew more slowly in CX-hE group and their sizes were markedly smaller than those of Ad-hE group(P
