High phosphorus foods combined with 5/6 nephrectomy for establishing rat hyperparathyroidism model secondary to chronic renal failure
- VernacularTitle:高磷饮食+5/6肾切除制备慢性肾衰竭继发甲状旁腺功能亢进大鼠模型
- Author:
Dan LI
;
Xuezhi ZHAO
- Publication Type:Journal Article
- Keywords:
kidney failure,chronic;
hyperparathyroidism,secondary;
high phosphorus;
5/6 nephrectomy;
models,animal
- From:
Academic Journal of Second Military Medical University
2000;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a rat hyperparathyroidism model secondary to chronic renal failure,so as to lay a foundation for studying the mechanism and treatment of secondary hyperparathyroidism.Methods: Thiry-six male Sprague-Dawley rats,weighing 200-250 g,were randomly divided into 6 groups: 5/6 nephrectomy plus high phosphorus food group(STNx + HP),5/6 nephrectomy plus low phosphorus food group(STNx + LP),5/6 nephrectomy plus normal phosphorus food group(STNx + NP),sham operation plus high phosphorus food group(Sham + HP),sham operation plus low phosphorus food group(Sham + LP),and sham operation plus normal phosphorus food group(Sham + NP).Nephrectomy was performed in 2 steps.Serum phosphorus levels(P) and iPTH levels were detected at day 7 pre-operation and day 7,14,and 21 after the second operation.The kidneys,thyroid glands and parathyroid glands complex underwent pathological analysis 4 weeks after operation.Results: Five patients survived in STNx+NP group,4 in STNx+LP group and 4 in STNx+HP group postoperatively.No death occurred in sham operated groups.Serum phosphorus levels of nephrectomy groups at different time points after operation were higher than those before operation and those of sham operation groups(P
