Preparation of total isomalabaricanes and determination of Stelletin B and Stellettin D in Stelletta tenuis Lindgren by high pressure liquid chromatography
- VernacularTitle:细薄星芒海绵总萜烯的制备及Stellettin B和Stellettin D的含量测定
- Author:
Wei XIONG
- Publication Type:Journal Article
- Keywords:
Stelletta tenuis Lindgren;
total isomalabaricanes;
chromatography,high pressure liquid
- From:
Academic Journal of Second Military Medical University
2000;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a method for determining the contents of Stellettin B and Stellettin D in total isomalabaricanes in different batches of Stelletta tenuis Lindgren.Methods: A high pressure liquid chromatography(HPLC) method was applied.The column YMC Pack-SIL(250 mm?4.6 mm,5 ?m) was used at room temperature,and the mobile phase was n-hexane∶ethyl acetate(75∶25) with a flow rate at 1.0 ml?min~(-1) for 30 min.UV detector wave length was at 360 nm.Results: The regression equation were:Y=52 181 633 X+957 495(r=0.996 9)for Stellettin B and Y=62 636 828 X-451 022((r=0.998 0)) for Stellettin D,with the linear range being 2.4-30 ?g and 2.0-32 ?g,correspondingly.The RSD values for accuracy test,stability test and repeatability test were 3.07%-4.76%.The average contents of Stellettin B and Stellettin D in total isomalabaricanes in different batches of Stelletta tenuis Lindgren were 59.93% and 30.98%,respectively.Conclusion: This HPLC method provides a fast,accurate and effective way to determine the active contents in total isomalabaricanes from Stelletta tenuis Lindgren.
