Effect of PKC? inhibitor on permeability injury of pulmonary microvascular endothelial cells monolayer induced by lipopolysaccharide
- VernacularTitle:蛋白激酶C?亚型抑制剂对脂多糖诱导肺微血管内皮细胞损伤的影响
- Author:
Zhongming ZHU
;
Gengyun SUN
- Publication Type:Journal Article
- Keywords:
protein kinase C;
lipopolysaccharides;
endothelial cells;
permeability
- From:
Chinese Pharmacological Bulletin
2003;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study the expression of protein kinase C(PKC) subtype PKC? in rat pulmonary microvascular endothelial cell(RPMVEC),and the effect of its inhibitor(Rottlerin) on LPS-induced RPMVEC monolayer permeability injury.Methods In cultured RPMVEC,Western blot and immunohistochemistry were used to identify the expression of PKC?.Micro-infiltrator was used to measure the changes of RPMVEC monolayer permeability coefficient(Kf) after exposure to LPS or LPS with Rotterin together.Result We found a high level of PKC? expression in cytoplasm.Rottlerin could obviously inhibit the increase of LPS-induced RPMVEC Kf value.Conclusion The activation of PKC is involved in the progress of LPS-induced RPMVEC injury.Furthermore,the inhibitor of PKC? could release LPS-induced RPMVEC Kf increase.
