Regulatory effect of iptakalim hydrochloride on excess expression of adhesive molecules in endothelial cells injury with high concentration of D-glucose
- VernacularTitle:盐酸埃他卡林对高糖致内皮细胞粘附分子基因过度表达的调节作用
- Author:
Zhibian DUAN
;
Hai WANG
- Publication Type:Journal Article
- Keywords:
Iptakalim hydrochloride;
D-glucose;
endothelial cells;
cell adhesive molecules
- From:
Chinese Pharmacological Bulletin
2003;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the regulatory effects of Iptakalim hydrochloride(Ipt) on excess expression of monocyte chemoattractant protein-1(MCP-1), intercellular cell adhesive molecule type-1(ICAM-1),vascular cell adhesive molecule1-1(VCAM-1) mRNA in bovine aortic endothelial cells cultured with high concentration D-glucose.Methods The endothelial cells were divided into three groups: control,model and Ipt group.The MCP-1,ICAM-1 and VCAM-1 mRNA was detected by reverse transcription-polymerase chain reaction (RT-PCR).Results Compared with control group,MCP-1,ICAM-1 and VCAM-1 mRNA expres sion of model group all increased after treatments with 25 mmol?L~(-1) D-glucose for 16 h,respectively.Compare with model group,the excess expression of MCP-1,ICAM-1 mRNA was blocked by 1～10 ?mol?L~(-1) Ipt added for 20 h in advance,respectively but no significant effect was found in the expression of VCAM-1 mRNA.Conclusion High concentration of D-glucose induced excess expression of MCP-1,ICAM-1 and VCAM-1 mRNA,respectively.The excess expression of MCP-1,ICAM-1mRNA is inhibited by Ipt.
