Gender-based differences in genistein metabolism in rat liver microsomes
- VernacularTitle:染料木黄酮在雌雄大鼠肝微粒体中的代谢差异
- Author:
Rutao WANG
;
Siyuan ZHOU
;
Qibing MEI
;
Xingling DAI
;
Yuhua RAN
;
Zhenguo LIU
- Publication Type:Journal Article
- Keywords:
genistein;
liver microsomes;
CYP1A2
- From:
Chinese Pharmacological Bulletin
2003;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study the gender-based difference in genistein metabolism in rat liver microsomes in vitro.Methods Liver microsomes was obtained from male and female rats.The optimized system of enzyme catalytic reaction of genistein in rat liver microsomes was set up.The reaction velocity of genistein in female and male rat liver microsomes was assessed by incubating genistein with CYP1A2 antibody or specific CYP1A2 inhibitor furafylline,and the percentage of the relative metabolism of genistein of CYP1A2 was derivated by using the data of the reaction velocity.Results The metabolism of genistein by microsomes was inhibited by CYP1A2 antibody(1 ∶400)after incubation for 30 min.The percentage of the control metabolism of genistein of microsomes in male and female rat were 20.95%?2.13% and 13.73%?1.26%respectively(P
