Effect of curcumin on sterol regulatory report system in HEK-293
- VernacularTitle:姜黄素对293细胞固醇调控报告系统的作用
- Author:
Ying QIAN
;
Chunlei FAN
;
Xiaobing DOU
;
Lingfeng HU
;
Xingde WO
- Publication Type:Journal Article
- Keywords:
HEK-293;
sterol regulatory element-1;
low density lipoprotein receptor;
green fluorescence protein;
curcumin
- From:
Chinese Pharmacological Bulletin
2003;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the lipid-lowering mechanism of Chinese Medicine curcumin.A sterol regulatory report system of LDLR gene expression in HEK-293 cell line was established.Method A green fluorescent protein(GFP) gene was constructed downstream of the sterol regulatory element-1(SRE-1) and the constructed plasmid was introduced into HEK-293 cell line.This SRE-GFP transfected 293 cells were treated with different concentration of curcumin for 2 days.The expression of GFP in the cells was measured by a fluorescence microscope and Flow Cytometry.Results The expression of GFP in SRE-GFP transfected 293 cells was obviously induced by curcumin.Conclusion Curcumin may positively affect the expression of LDLR gene by its influence on SRE-1.
