Protective effect of 5-lipoxygenase inhibitor zileuton on focal cerebral ischemia-reperfusion injury in rats
- VernacularTitle:5-脂氧合酶抑制剂zileuton对大鼠局灶性脑缺血/再灌注损伤的保护作用
- Author:
Lin XU
;
Juan YU
;
Ying LIU
;
Chonghong CHEN
- Publication Type:Journal Article
- Keywords:
zileuton;
focal cerebral ischemia-reperfusion injury;
lipid peroxidation;
inflammation;
NO
- From:
Chinese Pharmacological Bulletin
2003;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the protective effect of zileuton,a 5-lipoxygenase inhibitor,on focal cerebral ischemia-reperfusion injury in rats.Methods The right middle cerebral artery of the rat was occluded by inserting a thread through internal carotid artery for 2 h,and then reperfused for 24 h.Zileuton(10,50 mg?kg~(-1)) was orally administered 2 h before ischemia and 0,5,10 h after reperfusion.After 24 h reperfusion,the content of malondialdehyde(MDA) and nitric oxide(NO),the activities of glutathion peroxidase(GSH-PX),myeloperoxidase(MPO) and nitricoxide synthase(NOS) were measured.Results Compared with vehicle group,the infarct size,content of NO and NOS of the brain were significantly reduced in 10 and 50 mg?kg~(-1) zileuton groups;zileuton 50 mg?kg~(-1) also reduced the content of MDA,increased the activity of GSH-PX,and inhibited the increase of MPO in brain tissue.Conclusion Zileuton possesses the neuroprotective effects on focal cerebral ischemia-reperfusion injury in rats.
