Comparison between effects of dexamethasone and PPAR? agonist pioglitazone on intracellular cytokines expression of T_H1/T_H2 and the mechanism of that in asthma model
- VernacularTitle:地塞米松与吡格列酮对哮喘T_H1/T_H2细胞因子作用机制的比较
- Author:
Shixiu PAN
;
Tianshu ZENG
;
Tianpen CUI
;
Lihua HU
;
Jianmin WU
- Publication Type:Journal Article
- Keywords:
dexamethasone;
pioglitazone;
PPAR?;
asthma;
cytokine;
transcription factor
- From:
Chinese Pharmacological Bulletin
2003;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study the effects of dexamethasone(DXM)and peroxisome-proliferation activated receptor?(PPAR?)agonist pioglitazone on intracellular cytokines expression of T_H1/T_H2 and the mechanism of that in asthma model,and compare the difference between two drugs.Methods 24 Balb/c mice were divided into 4 groups randomly: control group,asthma group,DXM group,and pioglitazone group,with 6 mice in each group.The expression of T-bet and GATA3 of lung were detected using western blot,and the intracellular cytokines interluekin 4 and interferon ? expressions of CD~(4+)Tcell were measured using flowcytometry.Results Compared with control group,the level of the expression of T-bet of lung in asthma group increased significantly(P0.05),while both of GATA3 and T-bet decreased after DXM treatment,and the decreasing degree of GATA3 was more than that of T-bet.The results of flowcytometry indicated that the ratio of intracellular cytokines IL-4/IFN? of CD~(4+) T cell in asthma group was much higher than that of control group(P
