The effects of SSTG drug serum on Caclium-CaM-CaMPKⅡ signal system in cardiomyocytes injury induced by hypoxia and reoxgyenization
- VernacularTitle:双参通冠方药物血清对缺氧/复氧心肌细胞Ca~(2+)-CaM-CaMPKⅡ信号系统的影响
- Author:
Xiao HAN
;
Jianxun LIU
- Publication Type:Journal Article
- Keywords:
serum pharmacology of chinese herbal medicine;
hypoxia and reoxygenation;
calcium;
CaM;
CaMPKⅡ?
- From:
Chinese Pharmacological Bulletin
2003;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore the effects of Shuangshen tongguan(SSTG) drug serum on Calcium-Calmodulin(CaM)-Calcium/CaM dependent kinaseⅡsignal system in cardiomyocytes injury induced by hypoxia and reoxgyenization.Methods The cardiomyocytes were deprived of oxygen and glucose to mimic hypoxia reoxygenation injury.Intracellular calcium concentration,calmodulin(CaM) and calcium/calmodulin dependent kinaseⅡ?(CaMPKⅡ?) mRNA were measured by fluorospectrophotometry and reverse transcription-polymerase chain reaction(RT-PCR)respectively.Results After hypoxia/reoxygenation,intracellular calcium concentration,CaM and CaMPKⅡ? expression were enhanced(P
