Determination of Ganciclovir concentration in dog serum by RP-HPLC
- VernacularTitle:反相高效液相色谱法测定犬血清中更昔洛韦的浓度
- Author:
Wei WU
- Publication Type:Journal Article
- Keywords:
HPLC;
Ganciclovir;
serum concentration
- From:Journal of Third Military Medical University
2003;0(13):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for determining Ganciclovir concentration in dog serum by RP-HPLC.Methods The serum was determined by high performance liquid chromatography.A C_(18) column(5 ?m,250?4.6 mm) was applied.The mobile phase was 15 mmol/L potassium phosphate buffer(pH 2.5) containing 0.25% acetonitrile.The flow rate was 1.75 ml/min.The detector set at 254 nm.Column tempetature was 40 ℃.Results The linearity was excellent over the range of 0.050 25-100.5 ?g/ml;The methodological recovery ranged from 96.565% to 105.718%.The within-day and between-days relative standard deviations(RSD) were less than 1.400% and 3.800% respectively.Conclusion The elaborated method is sensitive,precise and can be used in the pharmacokinetic studies.
