Relationship between HCV chronic infection and expression of IL-8,IP-10,Mig
- VernacularTitle:丙型肝炎病毒慢性感染与IL-8、IP-10、Mig表达相关性研究
- Author:
Jian WANG
- Publication Type:Journal Article
- Keywords:
hepatitis C;
chemokine;
mRNA;
real-time quantitative polymerase chain reaction
- From:Journal of Third Military Medical University
2003;0(13):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the relationship between HCV chronic infection and expression of CXC chemokines mRNA.Methods The levels of IL-8,IP-10 and Mig mRNA in peripheral blood mononuclear cells(PBMC) of chronic hepatitis C patients were detected by real-time quantitative polymerase chain reaction(RT-PCR) before,and 3,6 months after combined therapy of interferon-?(IFN-?) and Ribavirin.The rate of chemokine/GAPDH was regarded as the extreme level of chemokine.Results The mean level of HCV-RNA and expressive levels of IL-8,IP-10,Mig mRNA in peripheral blood of HCV chronic infection were 2.09?10~(6)copies/ml,and(1.243 7?0.071 2),(0.682 8?0.073 8),(0.668 3?0.075 4),with significant difference with those of normal controls(P0.05).Conclusion The expressions of IL-8,IP-10 and Mig mRNA in PBMC are high in patients with chronic hepatitis C,and associated with the replication level of HCV,and less associated with the gene type of HCV.IFN-? can effectively restrain the replication of HCV and down-regulate the levels of IL-8,IP-10 and Mig mRNA near to normal level in patients with chronic hepatitis C.IFN-? plays an important role in down-regulating inflammation response and controlling the development of the patients' condition.
