Effect of Hypoxia on the Expression of Hypoxia Inducible Factor 1? and Vascular Endothelial Growth Factor in Lung of Mice
- VernacularTitle:低氧对小鼠低氧诱导因子-1?和血管内皮生长因子表达的影响
- Author:
Yongxia BAO
;
Fuzhen LU
;
Yingjun MA
- Publication Type:Journal Article
- Keywords:
Angiogenesis;
HIF-1?;
VEGF;
Lung;
Mice
- From:
Chinese Journal of Practical Internal Medicine
2006;0(14):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of hypoxia inducible factor-1?and VEGF in lung of mice during hypoxia and its relationship with angiogenesis.Methods The mice were divided into two group(hypoxia group and control group).The hypoxia group mice were placed in a hypoxia chamber with 10%,7%,or 5%O2 respectively for 3,6,9 days.Immuno- histochemical staining technique was used to examine HIF-1?,VEGF and MVD.The p rotein expression of HIF-1? and VEGF were observed by Western blot.Results HIF-1?proteins didn't express and VEGF proteins expressed weakly in control group mice;Compared with the controls,the expression of HIF-1?and VEGF increased dramadically.There were significant differences between the hypoxia groups and the control group;Positive relationships were found between the expression of HIF-1?,VEGF and MVD.Conclusion HIF-1?/VEGF pathway may play an important role in angiogenesis of hypoxia in the lung of mice.
