Effect of adhesion of Lactobacillus to enterocyte-like HT-29 cells on filamentous cytoskeleton
- VernacularTitle:乳杆菌黏附肠上皮样细胞HT-29对微丝骨架的影响
- Author:
Bin WANG
;
Jing YUAN
;
Hong WEI
- Publication Type:Journal Article
- Keywords:
Lactobacillus;
Adhesion;
Permeability;
F-actin
- From:Parenteral & Enteral Nutrition
1997;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of adhesion of Lactobacillus reuteri JCM1081 to enterocyte-like HT-29 cells on filamentous cytoskeleton.Methods:Cultured human intestinal enterocyte-like HT-29 cells were used.Cell permeability was examined in vitro by Evans blue labeled albumin and Examination of F-actin was conducted by direct immuno-fluorescence labeling using fluorescein labeled phalloidin.Results:Lactobacillus reuteri JCM1081 did not alter cell integrity and prevented the increase in permeability induced by enteropathogenic Escherichia coli infection.The distribution of F-actin were also not altered.Conclusion :Lactobacillus reuteri JCM1081 exerts a protective effect against the filamentous cytoskeleton rearrangement and permeability lesions promoted by enteropathogenic Escherichia coli infection.
