Preparation and biological characteristics of MTX and VCR co-encapsulated erythrocytes
- VernacularTitle:甲氨蝶呤与长春新碱双载红细胞的制备及其生物学特性
- Author:
Xuan WANG
;
Baohua QIAN
;
Zhanshan CHA
- Publication Type:Journal Article
- Keywords:
erythrocytes;
drug carriers;
methotrexate;
vincristine;
biological characteristics
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the method and feasibility of preparing MTX+VCR-loaded erythrocytes using healthy adult erythrocytes. Methods We used the modified hypotonic pre-swelling and isotonic resealing technique to prepare MTX+VCR-loaded erythrocytes. The loading parameters and releasing rate were evaluated with high penformance liquid chromatograph (HPLC) and auto-cytoanalysis. We observed the osmotic fragility of MTX+VCR-loaded erythrocytes conventionally. The change in innate immune activity of co-encapsulated erythrocytes was measured by counting the rosette rate of MTX+VCR-loaded erythrocytes adhering to S180. Results The concentration of MTX showed significant influence on the co-encapsulation efficiency (P0.05). Conclusions The preparation and biological characteristics of MTX+VCR-loaded erythrocytes were not different from those of mono-loaded erythrocytes, and erythrocytes were practically capable to be used as carriers for co-encapsulation of MTX and VCR.
