Effects of simulated weightlessness on eNOS expression in pulmonary artery in rat
- VernacularTitle:模拟失重对大鼠肺动脉eNOS表达的影响
- Author:
Congjun HAO
;
Changting LIU
;
Junfeng WANG
- Publication Type:Journal Article
- Keywords:
weightlessness simulated;
pulmonary artery;
nitric oxide;
orthostatic intolerance
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of simulated weightlessness on eNOS expression in the pulmonary artery in rat, and to investigate the mechanism of changed reactivity of pulmonary artery during simulated weightlessness. Methods The experiments were conducted in 16 male Wistar rats, and they were divided into 2 groups: suspension group and control group, 8 rats for each. Tail-suspension (-30?) was carried out for 14d to simulate weightlessness. eNOS protein expression and NO content in pulmonary artery were determined using Western blot analysis and nitrate enzyme-reduction method, respectively. Results Compared with control group, eNOS expression in the rat pulmonary artery increased significantly during simulated weigtlessness. The relative optical density as a result of scanning was 247?2.60, and that of the control was 141?1.72. There were significant differences between the two groups (P
