The effect of sodium selenite on heat induced aggregation of lens crystallin
- VernacularTitle:亚硒酸钠对晶状体蛋白质热凝聚的影响
- Author:
Baoyi ZHU
;
Yongqiang WANG
;
Hong YAN
- Publication Type:Journal Article
- Keywords:
sodium selenite;
crystallins;
heat aggregation
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of sodium selenite on heat aggregation of lens crystallin. Methods 12 fresh and intact bovine lenses were incubated with different concentrations of sodium selenite for 72 hours. The lenses of the control group were incubated without sodium selenite for the same length of time. After removing the capsule homogenate, the ?-, ?- and ?-crystallin from the lenses were separated by gel filtration chromatography. After the separation and purification, the crystallin were heated at 60℃ for 30 minutes. Then the absorption value at 360nm was measured again. The difference in the percentage of the absorption value after aggregation was analyzed. Results Comparing with the control group, the lenses incubated with sodium selenite showed earlier opacification in a concentration-dependent manner. After the separation by gel filtration chromatography, the ?-crystallin peak showed an antelocation on the chromatogram, suggesting that there was some high molecular aggregation formation. The aggregation of selenite-treated fractions showed a concentration-dependent increasing absorption compared with normal control portions (P
