Changes of SHP-1 mRNA and protein of bone marrow cells of mice with leukemia induced by ?-ray irradiation
- VernacularTitle:?射线诱发的白血病小鼠骨髓细胞SHP-1 mRNA及蛋白表达变化的研究
- Author:
Dingde HUANG
;
Qi CHEN
;
Ling HAN
;
Jianming CAI
- Publication Type:Journal Article
- Keywords:
radiation carcinogenesis;
signal transduction;
SHP-1;
BALB/c mouse;
leukaemia
- From:Journal of Third Military Medical University
2002;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate alteration of SHP-1 mRNA and protein of bone marrow cells of leukemia mice after ?-ray exposure.Methods A total of 318 BALB/c mice were exposed to ~(60)Co ?-ray once a week for 4 weeks,and the total dose of ?ray received by mice was 7.00 Gy.By pathological examination,39 mice developed thymic lymphoma,14 acute lymphocytic leukemia,21 T-lymphoblast leukemia/lymphoma,1 spiroma,4 malignant yolk sac tumor.Exposed to ?-ray,the mice that developed leukemia or were free of canceration were used in our study(n=10 in each group),and another 10 mice free from irradiation served as control.SHP-1 mRNA and protein in femoral bone marrow cells were detected by real-time fluorescence quantitative PCR(FQ-PCR) and Western blotting respectively.Results SHP-1 mRNA in leukemia mice was(5.26?2.14),significantly higher than that of mice free of canceration(3.68?1.27) and controls(2.95?1.09)(P
