Study on Inhibition of C.Chrysantha on DEN Induction of Murine Liver Cancer
- VernacularTitle:金花茶对二乙基亚硝胺致大鼠肝癌抑制作用研究
- Author:
Xiaoxian DUAN
;
Xiaolan TANG
;
Jianjia SU
- Publication Type:Journal Article
- Keywords:
Camellia Chrysantha;
DEN;
HCC
- From:
Journal of Medical Research
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective Using a short-term in vivo laboratory model of murine liver cancer induced with diethylnitrosamine (DEN),we inspected the impact of 5% C.Chrysantha leaves and 5% concentrations on induction of liver cancer with DEN.Methods Laboratory Winstar rats,all being male and SPF grade,were administered with the liver cancer initiator DEN(200?g/kg,ip,once only),then with murine food containing 0.015% 2-AAF (2-Acetaminoflueren) for 2 weeks,following an excision of the augmented parts as a selective promoting program that speeded up the proliferation of precancerous foci from the initiated hepatocytes.On the beginning of this experiment,5% C.Chrysantha leaves and 5% concentration-contained food were given to the trail rats; in contrast,normal food to the control.Rats were killed 3 days after feeding of 2-AAF-contained food.Targeting part of liver were collected for ?-glutamyltranspeptidase (?-GT) staining,and numbers and areas of?-GT positive foci per cm2 of the liver area were calculated using a software designed by us.Results Numbers and areas of?-GT positive foci of the trail are significantly less than those of the control.Conclusion 5% C.Chrysantha leaves and 5% concentrations inhibit the DEN induction to liver cancer.
