EFFECTS OF SELENIUM ON GENE EXPRESSION OF PHOSPHOLIPASE D GENE IN LIVER IN DIABETIC RATS
- VernacularTitle:硒对糖尿病大鼠肝脏磷脂酶D基因表达的影响
- Author:
Yuntang WU
;
Zhong SUN
;
Xia WANG
;
Xiaoyong LIU
;
Zhuangzhi ZHOU
;
Yongming WANG
- Publication Type:Journal Article
- Keywords:
selenium;
diabetes;
gene expression;
RT-PCR;
PLD
- From:
Acta Nutrimenta Sinica
1956;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the regulation of selenium on gene expression of phospholipase D (PLD) gene in relation to liver metabolism of diabetic (DM) rats. Method: Differential display cDNA fragments were isolated from former research project in diabetic rats which were supplemented orally with 50 g/kg bw d selenium for 60d, and were cloned, sequenced, and the homology was analyzed. RT-PCR was made using the primers designed according to the sequences of cDNA fragments. Results: In differential display cDNA fragment, Se-4 and PLD were homologous with sequence identities of 100%.The expression levels of PLD mRNA in DM group and DM+Se group was higher obviously than that of NC group(P
