SEPARATION OF ORGANIC ACIDS BY CAPILLARY ZONE ELECTROPHORESIS AND ELECTROCHEMICAL DETECTION IN FRUIT JUICES
- VernacularTitle:毛细管区带电泳分离/电化学检测果汁中有机酸
- Author:
Ning GAN
;
Congxin GE
- Publication Type:Journal Article
- Keywords:
high performance capillary electrophoresis (HPCE);
-CD;
electrochemical detection;
organic acids;
fruit juice
- From:
Acta Nutrimenta Sinica
1956;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: The method for separation by capillary zone electrophoresis (CZE) and electrochemical determination of organic acids in fruit juices was developed. Method: In this system, 0.2mmol/L cetylpyridinium bromide (CPB) was used as an electro osmotic flow (EOF) modifier to reverse the direction of EOF. cyclodexfrin ( -CD) was added into running buffer to improve the separation efficiency. Then, the optimal separation conditions were achieved and successfully employed to separate six organic acids, including oxalic, malic, tartaric, succinic, fumaric acid and citric acids. One nano palladium modified carbon paste electrode was used for determination. Results: The calibration curves for all organic acids studied were linear with 2-3 orders (1 10–6-1.0 10–3 mol/L) of magnitude and all the detection limits (S/N=3) were below 2 mol/L. The RSD was between 1.05%-2.15% , and recovery was 93.4% . Conclusion: CZE separation combined with electrochemical detection is suitable for simultaneous determination of organic acids in fruit juices quickly with high sensitivity and selectivity.
