Effect of levcromakalim on ET-1 induced proliferation and expression of protein kinase C in vascular smooth muscle cells
- VernacularTitle:拉马克啉对内皮素-1诱导的大鼠血管平滑肌细胞增殖及蛋白激酶C表达的作用
- Author:
Wenxin HUANG
;
Biwen MO
;
Yaozhong YANG
;
Chunsheng FANG
;
Zhonghua XIA
;
Dihua PAN
;
Tongtong XU
;
Xinling MO
- Publication Type:Journal Article
- Keywords:
levcromakalim;
vascular smooth muscle cells;
proliferation;
protein kinase C
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2002;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To observe the effect of levcromakalim(Lev) on endthelin-1(ET-1) induced proliferation and the expression of protein kinase C(PKC) in cultured rat vascular smooth muscle cells(VSMCs).METHODS: VSMCs were isolated from rat aorta and were cultured with different concentrations of Lev in vitro after stimulated to proliferation by ET-1(10~(-8)(mol?L~(-1))).Vitality of VSMCs was detected by MTT;DNA replication was evaluated by 3H-TdR method.Flow cytometry was used to analyze cell cycle and apoptosis.The expression of PKC? protein and mRNA were determined by Western blot and reverse transcription-polymerase chain reaction(RT-PCR).RESULTS:Vitality of VSMCs and 3H-TdR intermingle rate were decreased(P
