Establishment and optimization of two-dimensional gel electrophoresis for separation of human kidney phosphoproteome
- VernacularTitle:人肾组织磷酸化蛋白质组二维凝胶电泳的建立与优化
- Author:
Yawei LIU
- Publication Type:Journal Article
- Keywords:
proteomics;
phosphoproteome;
two-dimensional gel electrophoresis
- From:
Academic Journal of Second Military Medical University
1985;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To separate human kidney phosphoproteome by two-dimensional gel electrophoresis.Methods: The phosphorylated proteins from human kidney tissues were enriched with phosphate metal affinity chromatography(PMAC) resin.After being concentrated and desalted,the samples were separated by isoelectric focusing on first dimension and SDS electrophoresis on second dimension.Results: The phosphorylated proteins were successfully extracted from human kidney tissues and were separated by two-dimensional gel electrophoresis.Conclusion: Phosphoprotein enrichment technique combined with two-dimensional gel electrophoresis is an effective approach to study phosphoproteome,laying a foundation for further investigation of human kidney phosphoproteins.
