Myeloma protein-pusled dendritic cells induced anti-myeloma cells activity
- VernacularTitle:M蛋白致敏树突状细胞诱导的抗骨髓瘤细胞效应
- Author:
Junmin LIU
;
Yufeng LI
;
Henbao XUAN
;
Yuanyuan LI
;
Zhikui DENG
- Publication Type:Journal Article
- Keywords:
multiple myeloma;
myeloma protein;
dendritic cells;
T-lymphocytes
- From:
Academic Journal of Second Military Medical University
1985;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the anti-myeloma cells specific immune activity induced by myeloma protein-pusled dendritic cells(DCs).Methods: DCs were obtained from peripheral blood(PB) of multiple myeloma(MM) patients after co-culture with GM-CSF,IL-4 and TNF-?.The phenotype of DCs was examined by flow cytometry.Host lymphocytes were stimulated with DCs pulsed with myeloma protein acquired from serum of MM patients on DEAE-celluose;lymphocytes treated with unpulsed-DC served as control.[~(3)H]-thymidine incorporation and 4 h ~(51)Cr-release assay were used to detect the proliferation and cytotoxicity against different targets(auto-myeloma cells,U266 cells) by T lymphocytes.Results: In vitro proliferation and cytotoxicity of T lymphocytes activated by myeloma protein pulsed-DCs were greater than those of T lymphocytes in the control group(P
