Comparison the effect of arachidonic acid and its′ metabolites on rabbit pulmonary arterial rings
- VernacularTitle:花生四烯酸及其3种代谢产物对兔肺动脉环作用的比较
- Author:
Shouli GUO
;
Qian LI
;
Yifei ZHANG
;
Ye LIU
;
Daling ZHU
- Publication Type:Journal Article
- Keywords:
arachidonic acid;
8(S),15(S)-DiHETE;
15-HETE;
15-KETE;
pulmonary arterial ring;
hypoxia
- From:
Chinese Pharmacological Bulletin
1986;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim The purpose of this study was to compare the effect of AA、15-HETE、15-KETE and 8(S),15(S)-DiHETE on hypoxic and normoxic rabbit pulmonary arterial rings and to discuss their roles in the formation of PA hypertension.Methods Twelve neonatal rabbits were randomly divided into two groups(n=6):the normoxic group breathing fresh gas(FiO_2 =21%) and the hypoxic group breathing hypoxic gas(FiO_2=10%).After 9 days,they were anesthetized with pentobarbital sodium,and the chest was opened for removal of the heart and lungs en bloc,then PA rings(0.5~1.5 mm in diameter and 3 mm length) were prepared.We used tension studies of PA rings to observe the effect of AA、15-HETE、15-KETE and 8(S),15(S)-DiHETE on hypoxic and normoxic rabbit pulmonary arterial rings.Results ① AA、15-HETE、15-KETE and 8(S),15(S)-DiHETE constricted normoxic rabbit PA rings in a dose-dependent manner,respectively.Constriction of normoxic rabbit PA rings induced by 15-KETE、8(S),15(S)-DiHETE was significant,but that of AA、15-HETE wasn′t significant.② AA、15-HETE、15-KETE and 8(S),15(S)-DiHETE increased hypoxic rabbit PA rings' tension.Constriction of AA、15-HETE on rabbit PA rings in hypoxic group was significantly greater than that of normoxic group(P0.05).③ Constriction of 15-KETE on rabbit PA rings in normoxic group was significantly greater than that of hypoxia group(P
