Effects of achyranthes bidentata polysaccharides on eosinophil and mast cells in airway of young as thmatic rats
- VernacularTitle:牛膝多糖对幼年哮喘大鼠气道壁嗜酸性粒细胞和肥大细胞的影响
- Author:
Haiyan ZHANG
;
Jun FANG
;
Xiaoguang HU
;
Aimin HUANG
- Publication Type:Journal Article
- Keywords:
asthma;
eosinophil;
mast cell;
apoptosis;
degranune;
achyranthes bidentata polysaccharides
- From:
Chinese Pharmacological Bulletin
1986;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate changes of eosinophil(EOS) and mast cells(MC) in airway of young asthmatic rats and to evaluate of achyranthes bidentata polysaccharides(ABPS) on it.Methods Fifty Sprague-Dawley(SD) rats were randomly divided into five groups,ten rats per group:asthmatic group(A),control group(C),pretreatment groups with ABPS which was done according to three different schedules:consecutively 3 days at sensitization(T_1),at challenge(T_2) or both of the two periods(T_3).Asthmatic rats were induced by intraperitioneal sensitization and challenged with nebulized ovalbumin(OVA).Lungs were embeded in paraffine and sliced,Hematoxylin and eosin(HE) staind tobuidine blue restained and TUNEL method after animals were executed,then the quantity and degranulation phenomenon of EOS and MC and the apoptosis of EOS in airway were observed.Results ① The number of inflammatory cells and EOS in the airway was significantly increased in asthmatic group but reduced in group T_1 and T_3(P
