Protective effect of FMCL on the apoptosis of PC12 cells induced by H_2O_2
- VernacularTitle:山楂叶总黄酮对H_2O_2诱导PC12细胞凋亡的保护作用
- Author:
Yingshi JI
;
Hong LI
;
Shijie YANG
- Publication Type:Journal Article
- Keywords:
FMCL;
PC12 cells;
H_2O_2;
apoptosis
- From:
Chinese Pharmacological Bulletin
1986;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investige the protective effect of FMCL on the apoptosis of PC12 cells induced by H_2O_2 and its mechanisms.Methods Apoptosis of PC12 cells induced by H_2O_2 at the concentration of 0.5 mmol?L~(-1).The cell activity was determined by MTT.The morpholog changes were observed by invert microscope.DNA ladder was observed through agarose gel electrophoresis.The subdiploid peaks showing cell apoptosis rate and △?m were detected by flow cytometry.Results Compared with those of the Model group the cell activity of FMCL 100、30 mg?L~(-1) group reinforced,the injury of the cell relieved.The apoptosis rate of those two groups decreased,but the△?m and the cell activity increased in a dose-dependent manner.Conclusion FMCL can inhibit the apoptosis of PC12 induced by H_2O_2 which might be correlated with the increasion of △?m.
