The interference of Cd~(2+) with Fura-2 fluorescence in measurement of free cytoplasmic Ca~(2+)
- VernacularTitle:Fura-2荧光测定胞质Ca~(2+)实验中注意Cd~(2+)的荧光干扰作用
- Author:
Zheng ZHANG
;
Bingxiang CAI
;
Qinying QIU
;
Jiaguo ZHOU
;
Yongyuan GUAN
- Publication Type:Journal Article
- Keywords:
Cd~(2+);
Fura-2;
fluorescence hypersensitivity;
PC12 cells
- From:
Chinese Pharmacological Bulletin
1986;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the blockade of cadmium on store operated calcium channels and the fluorescence interference of cadmium with Fura-2.Methods PC12 cells were used to determine the intracellular calcium concentration [Ca~(2+)]i indicated by change in Fura-2 fluorescence ratio(F_(340)/F_(380)).Results Introduction of cadmium induced a significant increase in Fura-2 fluorescence ratio following thapsigargin-evoked calcium entry;Besides,cadmium gave rise to a remarkable elevation in Fura-2 fluorescence ratio following breakdown of the plasma membrane by triton,a detergent.The Fura-2 fluorescence was increased in the presence of cadmium and Fura-2/AM,simultaneously in the absence of PC12 cells.Conclusion Cadmium can interfere with the Fura-2 fluorescence.
