Induction of extracellular hydroxyl radical in striatum of rats by intracerebral perfusion of 6-OHDA
- VernacularTitle:建立脑内灌流6-OHDA所致大鼠纹状体细胞外液羟自由基升高的模型
- Author:
Danqiao WANG
;
Wei WANG
;
Dezhong ZHAO
- Publication Type:Journal Article
- Keywords:
microdialysis in brain;
hydroxyl radical;
6-hydroxy dopamine;
capture by salicylic acid
- From:
Chinese Pharmacological Bulletin
1986;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To provide an approach to drug filtration and the research into damage of brain cell caused by senile retrograde affection and oxidative stresses,a model of increased extracellular hydroxyl radical in striatum of rats was established by intracerebral perfusion of 6-hydroxy dopamine(6-OHDA).Method Microdialysis was used to establish the model,hydroxyl radicals captured by salicylic acid yielded 2,3-dihydroxy benzyl acid(2,3-DHBA) and 2,5-dihydroxy benzyl acid(2,5-DHBA) which were measured by HPLC-ED.Results After 75 minuets of the perfusion with 6-OHDA in brain of rats,the levels of 2,3-DHBA and 2,5-DHBA in the model group increased to 6.6 and 3.4 times compered with the control group respectively;and the 2,3-DHBA was still higher than that of the control group in the whole course of observation(P
