Changes of CD4~+CD25~+ and CD8~+CD28~- T regulatory cells in spontaneous tolerance of inbred rat orthotopic liver transplantation
- VernacularTitle:大鼠肝移植自发免疫耐受模型CD4~+CD25~+和CD8~+CD28~-调节性T细胞的变化
- Author:
Peng YOU
;
Yulan LIU
;
Guodong CHEN
- Publication Type:Journal Article
- Keywords:
Liver transplantation;
Immune tolerance;
T-lymphocyte subsets;
T-lymphocyte
- From:
Journal of Peking University(Health Sciences)
2004;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the roles of CD4+CD25+and CD8+CD28- T regulatory cells in spontaneous tolerance of inbred rats orthotopic liver Transplantation.Methods: The orthotopic liver transplantation models of spontaneous tolerance were established between BN (donor) and LEW (recipient) inbred rats whose survival time was more than 120 days, The expression of CD8+CD28- T and CD4+CD25+T cells in PBMC and spleen were determined by flow cytometry. CD8+CD28- T and CD4+CD25+T cells were sorted by immunomagnetic beads. The expressions of CD25, CD28, CD152 and CD45RO on CD8+CD28- T and CD4+CD25+T regulatory cells were also determined by flow cytometry. Results: Compared with control groups, the proportion of CD8+CD28-T regulatory cells in spontaneous models was significantly increased in peripheral blood mononuclear cells (PBMC) and spleen [PBMC:(30.2?5.8)% vs(16.4?6.1)%;Spleen(23.7?7.2)% vs(13.4?5.5)% P
