Analysis of the mitochondrial mutations of articular chondrocyte in temporomandibular joint osteoarthrosis
- VernacularTitle:颞下颌关节骨关节病髁突软骨细胞线粒体DNA突变的实验研究
- Author:
Zeqiang FANG
;
Xuchen MA
- Publication Type:Journal Article
- Keywords:
DNA,mitochondrial;
Osteoarthritis;
Mutation;
Electrophoresis
- From:
Journal of Peking University(Health Sciences)
2004;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To explore the significance of mitochondrial DNA mutations of articular chondrocytes in the rat model of temporomandibular joint osteoarthrosis (TMJOA). Methods:TMJOA models were created in left sides of TMJ of 15 SD rats by the partial resection of the articular disc.The experimental rats were killed 3 months after operation. After the chondrocytes culture, the entire mtDNA were amplified using 32 pairs of overlapping. DNA fragments showing different banding patterns between normal and experimental mtDNA by temporal temperature gradient gel electrophoresis were sequenced to identify the mutations. Results: Of the 35 heteroplasmic pattern PCR products, 42 novel mutations were found, Majority of the novel mutations were in the tRNA and D-loop regions. Conclusion:The mutations occurred in the mtDNA of TMJOA articular chondrocytes.
