Effect of L-arginine supplemented enteral nutrition on cellular immunological function in rats with severe head injury
- VernacularTitle:添加精氨酸的肠内营养对重度颅脑损伤大鼠细胞免疫功能影响的实验研究
- Author:
Jing ZENG
- Publication Type:Journal Article
- Keywords:
L-arginine;
Enteral nutrition;
Severe head injury;
Cellular immunity
- From:
Parenteral & Enteral Nutrition
2004;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objectives:The study was designed to observe the effects of L-arginine(Arg) supplemented enteral nutrition on cellular immunological function in rats with severe head injury.Method:The severe head injury of rats was produced by gas percussion.Thirty rats were divided randomly into 3 groups(n=10): normal control group,enteral nutrition(EN)group and EN+Arg group.Rats in groups EN and EN+Arg were supplemented with tyrosine at the does of 0.8g?kg~(-1)?d~(–1)and Arg at the dose of 0.8g?kg~(-1)?d~(–1)for 7days,respectively.Blood samples were collected after 7 days.PMN phagocytosis rate was observed by phagocytosis Candida albicans count.CD4~(+)cells and CD8~(+) cells were analyzed by SABC.The concentration of serum IL-2 level was measured by radioimmunoassay.Results:Decreased cellular immunolgical function was found on 7~(th) day after severe head injury.However,CD4~(+),CD4~(+)/CD8~(+),IL-2 level and PMN phagocytosis rate in EN +Arg group were better than that in EN group.There was significant difference in each immunological index(P
