Effect of 1,25-dihydroxyvitamin D_3 and Tamoxifen on cell cycle distribution and apoptosis of MDA-MB-231 cells transfected with ER? expression vector containing vitamin D response element
- VernacularTitle:1,25二羟维生素D_3联合他莫西芬对转染VDRE-Tk-ER?表达载体的MDA-MB-231细胞周期和凋亡的影响
- Author:
Haibin LANG
;
Mantian MI
- Publication Type:Journal Article
- Keywords:
1,25-dihydroxyvitamin D_3;
cell cycle;
apoptosis;
ER?;
Tamoxifen;
MDA-MB-231
- From:Journal of Third Military Medical University
2003;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of 1,25-dihydroxyvitamin D_(3) and Tamoxifen on cell cycle distribution and apoptosis of MDAMB-231 cells transfected with ER? expression vector containing vitamin D response element.Methods The 4 VDRE-Tk sequence of VDRE-pGL3 reporter vector was cloned into ER?/pcDNA3.1+ vector and then was transfected into MDA-MB-231 breast cancer cells.MDA-MB-231~(VDRE-ER?) breast cancer cells were treated with 1,25-dihydroxyvitamin D_(3) and Tamoxifen to observe their pro-apoptotic effect by TUNEL and to determine whether cell cycle progression could be repressed by FCM.Results As compared with control group,MDA-MB-231~(VDRE-ER?) cells at G_(0)/G_(1) phase increased profoundly after treated with 1,25-dihydroxyvitamin D_(3) and Tamoxifen for 72 h.Meanwhile,1,25-dihydroxyvitamin D_(3) and Tamoxifen synergistically induced the apoptosis of MDA-MB-231~(VDRE-ER?) cells.Conclusion Transfection of VDRE-Tk-ER? expression vector into ER?-negative breast cancer cells could effectively recover its responsiveness to Tamoxifen under the induction of 1,25-dihydroxyvitamin D_(3) by blocking the cell cycle progression and inducing apoptosis.
