Effects of rosiglitazone on the mRNA expressions of MMP-1、TIMP-1 and Collagen Ⅲ in rat kidney fibroblasts cultured in high glucose.
- VernacularTitle:罗格列酮对高糖环境下大鼠肾脏成纤维细胞MMP-1TIMP-1及Ⅲ型胶原mRNA表达的影响
- Author:
Zhaohui ZHENG
;
Haijian LI
;
Zhangsuo LIU
- Publication Type:Journal Article
- Keywords:
Fibroblasts;
Matrix metalloproteinases;
Collagen Ⅲ;
Rosiglitazone
- From:
Chinese Journal of Practical Internal Medicine
2000;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate into the protective effects of rosiglitazone on rat kidney fibroblasts(NRK)damaged by high glucose.Methods From Jan.2005 to Sep.2005,the NRK cells were cultured in vitro,and were divided into five groups:normal glucose group(NG,1 000 mg/L D-glucose),high glucose group(HG,4 500 mg/L D-glucose),HG+RGZ(5 ?mol/L),HG+RGZ(10 ?mol/L)and HG+RGZ(15 ?mol/L).The mRNA expressions of T MMP-1、TIMP-1 and Collagen Ⅲ were measured with RT-PCR.Results Compared with NG group,the mRNA expression of MMP-1 decreased markedly in HG group(P
