Effects of Leonurus heterophyllus sweet injection on myocardial cell apoptosis and proliferation activity in the diabetic cardiomyopathy rats.
- VernacularTitle:益母草注射液对糖尿病心肌病大鼠心肌细胞凋亡和增殖活性的作用研究
- Author:
Qi XU
;
Shenren CHEN
;
Lishu CHEN
- Publication Type:Journal Article
- Keywords:
Leonurus heterophyllus sweet;
Diabetic cardiomyopathy;
Apoptosis;
Proliferation
- From:
Chinese Journal of Practical Internal Medicine
2000;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of Leonurus heterophyllus sweet injection(LHS)on myocardial cell apoptosis and proliferation activity in the diabetic cardiomyopathy rats induced by STZ.Methods From March to September of 2004,STZ-induced DCM model was established.The diabetic rats were divided into three groups:untreated DCM group,treated group by LHS injection and normal control group.At the end of 16 weeks,myocardial tissue of the rats was collected as experimental material to examine apoptosis by using TUNEL,electromicroscopic changes(ultrastructure)and related gene expression(Fas,FasL,Bax,Bcl-2)as well as proliferating cell nuclear antigen(PCNA)with immunohistochemistry method.Results 1.Compared with the DCM group,only a few myotomes of cardiac myocytes in LHS-treated group were out of their positions.There was no myofibril melt and the mitochondria structure kept intact.2.Compared with the DCM group,the numbers of myocardial cell apoptosis were significantly decreased(P
