Construction of the recombinent retroviral vector rRV-hIL-4 and expression of rheumatoid arthritis in vitro.
- VernacularTitle:携带hIL-4逆转录病毒重组体的构建及在类风湿关节炎中体外表达的研究
- Author:
Ning ZHANG
;
Haixiang FU
;
Lili YANG
- Publication Type:Journal Article
- Keywords:
Rheumatoid arthritis;
Vector construction;
Human interleukin 4
- From:
Chinese Journal of Practical Internal Medicine
2000;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct recombinent retroviral vectors containsins the expression sequence of hIL-4,and detect the expression of target genes.Methods Design the primer with restrictive enzyme spot and amplify target gene by PCR from plasmid including hIL-4 gene.Clone the target gene into retroviral vector pLXSN,and identify the aquired plasmid by sequencing.Transfect human synoviocytes with acquired virus in vitro.Detect the protein expresssion by western blotting.Results We successfully constructed the recombinant retrovirus-rRV-hIL-4.The hIL-4 gene was transduced into human synoviocytes by recombinant retrovirus in vitro.The protein expression of genes was detected by Western blotting.Conclusion Recombinant retrovirus rRV-hIL-4 is constructed successfully.The hIL-4 gene is transduced successfully into the human synoviocytes in vitro and the transduced synoviocytes can express hIL-4 protein.
