Analyses of differentially expressed proteins of human synovial fibroblasts obtained from osteoarthritis
- VernacularTitle:人骨关节炎滑膜成纤维细胞差异蛋白质谱初步分析
- Author:
Ganping BAI
- Publication Type:Journal Article
- Keywords:
osteoarthritis;
synovioblast;
proteome;
two-dimensional electrophoresis
- From:Journal of Third Military Medical University
2003;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To understand the possible roles of some proteins expressed by human synovial fibroblasts in the pathogenesis of osteoarthritis by means of 2-DE and bioinformatics.Methods The difference of synovial fibroblasts protein expression between osteoarthritis patients and healthy controls was analyzed by 2-DE.The differential expression spots were identified by matrix-assisted laser desorption ionization-time-of-flight mass spectrometry(MALDI-TOF-MS),followed by bioinformatics analysis.Results Gel-image analysis revealed that there were 36 differential protein spots.A total of 15 differential protein spots were identified by MALDI-TOF-MS,of which 6 spots were much more intensive in osteoarthritis patients than normal ones.Conclusion These differential proteins based on bioinformatics analysis might be involved in synovitis in osteoarthritis patients.
