Adhesion of Lactobacillus protects the enterocyte-like cell HT-29 against the invasion of enteropathogenic E. coli
- VernacularTitle:乳杆菌黏附抑制致病性大肠杆菌对肠上皮样细胞侵袭的初步研究
- Author:
Bin WANG
;
Guorong DAN
;
Jing YUAN
- Publication Type:Journal Article
- Keywords:
lactobacillus;
enteropathogenic Escherichia coli;
inhibition;
adhesion;
invasion
- From:
Medical Journal of Chinese People's Liberation Army
1981;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the beneficial effect on enterocyte-like cells HT-29 through adhesion of Lactobacillus. Methods Using viable bacteria counting method to evaluate inhibition of Escherichia coli adhesion and invasion to enterocyte-like cells by incubation with Lactobacillus together. Cellular membrane permeability and cell viability were examined in vitro by LDH level and MTT method respectively. Results Adhesion and invasion of enteropathogenic Escherichia coli to enterocyte-like cells was inhibited by high adhesiveness strain Lactobacillus reuteri JCM1081. In vitro, no significant changes of the morphology, structure and function of the HT-29 cells after being treated with Lactobacillus alone for 3 hours, especially cellular LDH level, viability and membrane permeability. Lactobacillus reuteri JCM1081 did not alter cell integrity and prevented the increase in permeability induced by enteropathogenic Escherichia coli infection. Conclusion Lactobacillus reuteri JCM1081 interact with intestinal epithelial cell receptor to competitively inhibit the adhesion and invasion of enteropathogenic Escherichia coli to HT-29 cells by incubation and enhance the integrity of the cells.
