Construction and expression of Mtb8.4 gene vaccine and study on cellular immune response induced by the vaccine
- VernacularTitle:结核病Mtb8.4基因疫苗构建、表达及诱导的细胞免疫应答
- Author:
Hui LI
;
Rong LI
;
Sen ZHONG
- Publication Type:Journal Article
- Keywords:
tuberculosis;
Mtb8.4;
gene vaccine;
construction;
expression;
celluar immune response
- From:
Medical Journal of Chinese People's Liberation Army
1981;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepare Mtb8.4 gene vaccine and to study the cellular immune response induced by the vaccine. Methods The gene encoding Mtb8.4 from Mycobacterium tuberculosis was amplified by PCR and cloned into the eukaryotic expression vector pcDNA3.1 (+). C57BL/6N mice were vaccinated three times with Mtb8.4 gene vaccine at 3 weeks interval. Four weeks after the final inoculation, mice were sacrificed to assess cytokine response and CTL induction. Results The IFN-? and IL-2 titers were 787.317?45.586pg/ml and 319.953?57.978pg/ml in Mtb8.4 gene vaccine group, 1 486.540?39.600pg/ml and 767.043?50.269pg/ml in BCG group, respectively. The level of IL-4 in BCG group (90.580?10.998 pg/ml) increased significantly as compared to other groups (P
