Synergistic inhibitory effects of pirarubicin combined with hyperthermia on proliferation and invasion of gastric cancer cells
- VernacularTitle:吡柔比星与温热协同对胃癌细胞增殖和侵袭的抑制作用
- Author:
Xiaoguang WAN
;
Jun FU
;
Hang ZHENG
- Publication Type:Journal Article
- Keywords:
pirarubicin;
hyperthermia;
neoplasm invasiveness;
stomach neoplasms
- From:
Medical Journal of Chinese People's Liberation Army
1981;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the synergistic effects of pirarubicin (THP) combined with hyperthermia on proliferation and invasive potential of gastrointestinal cancer cells in vitro and to discuss the underlying mechanisms. Methods Gastric cancer cell line MGC-803 was established, and they were divided into 4 groups: control group (37℃), high temperature group (43℃), drug group (37℃+THP), and drug and high temperature group (43℃+THP). After THP and hyperthermia treatment, cell proliferative ability was determined by soft agar clonogenic assay, cell nuclear antigen PCNA was detected by flow cytometry, cyclin E mRNA level was assessed by reverse transcription PCR (RT-PCR). Cell invasion assay was assessed by using transwell chamber model. Results Strong synergistic inhibitory effects on clonogenic ability of MGC-803 cells were observed after MGC-803 cells were treated with pirarubicin in combination with hyperthermia (P
