Effects of different magnitudes of mechanical strain on proliferation and alkaline phosphatase activity in osteoblast in vitro
- VernacularTitle:不同大小机械牵张力对成骨细胞增殖及碱性磷酸酶的影响
- Author:
Lin TANG
;
Zhu LIN
;
Yongming LI
- Publication Type:Journal Article
- Keywords:
mechanical strain;
osteoblast;
proliferation;
alkaline phosphatase
- From:
Medical Journal of Chinese People's Liberation Army
1981;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of different magnitudes of mechanical strain on proliferation and alkaline phosphatase (ALP) activity in mouse osteoblast-like cells (MC3T3-E1). Methods MC3T3-E1 cells were subjected to 0%, 6%, 12%, 18% elongation for 24h and 48h by using multi-passage cell stress loading system respectively. MTT colorimetric method was used to assess the proliferation of the cell, ALP activity was detected by ALP assay kit. Results The proliferation of MC3T3-E1 cells was increased significantly 24h and 48h after mechanical strain treatment concomitant with increasing stretching force (P
