Effect of ?-estradiol and progesterone on the proliferation of endometrial epithelial cells in vitro
- VernacularTitle:雌激素与孕激素在兔子宫内膜上皮细胞体外增殖中的作用
- Author:
Cuimi DUAN
;
Haibin WANG
;
Hong JIANG
- Publication Type:Journal Article
- Keywords:
endometrium;
estrogens;
progesterone;
cell proliferation
- From:
Medical Journal of Chinese People's Liberation Army
1981;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective The purpose of this study was to investigate the effect of different level of sex steroids (?-estradiol and progesterone) on the proliferation of uterus endometrial epithelial cells. Methods Rabbit uterus endometrial epithelial cells were isolated by digestion of trypsin and centrifugation. The specificity and homogeneity of the endometrial epithelial cells cultured in media containing ?-estradiol and progesterone were determined by immunofluorescence staining of cytokeratin monoclonal antibody and the flow cytometric analysis. The different concentration of progesterone (P4) and ?-estradiol (E2) were added in media, the proliferative capability of the endometrial epithelial cells cells in vitro was determined indirectly by MTT assay. Results High purity endometrial epithelial cells (96%) were isolated using centrifugation method. The endometrial epithelial cells were examined by immunofluorescence staining. The endometrial epithelial cells were positive stained by anti-cytokeratin antibody. Immunofluorescence staining demonstrated that cytokeratin was detectable in the cytoplasm of epithelial cells. The cell growth curve showed that the proliferation of endometrial epithelial cells was not significantly changed by the treatment of E2 (100nmol/L) or P4 (100nmol/L) individually, but a combination of 100nmol/L E2 and 10nmol/L P4 could promote its proliferation greatly (P
