Effect of propofol on amino acid levels in cultured pc12 cells impaired by n-methyl-d-aspartate
- VernacularTitle:异丙酚对N-甲基-D-天冬氨酸致PC12细胞损伤时氨基酸水平的影响
- Author:
Henglin WANG
;
Zhuoqiang WANG
;
Jiangbei CAO
- Publication Type:Journal Article
- Keywords:
propofol;
excitatory amino acids;
N-methyl-D-aspartate;
PC12 cells
- From:
Medical Journal of Chinese People's Liberation Army
1981;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the influence of propofol on amino acid levels in cultured PC12 cells impaired by N-methyl-D-aspartate, and explore the possible protective action mechanism of anesthetic propofol. Methods The levels of amino acid were measured by high performance liquid chromatography (HPLC). Results After exposing to NMDA 300?mol/L for 4h, the release of glutamate levels from PC12 cells was increased significantly, whereas the release of glutamine, aspartate, and glysine levels remained unchanged. In the presence of propofol 12.5, 125?mol/L for 4h, the levels of glutamate decreased significantly (P
