Expression of CD46, CD55, and CD59 on primary murine astrocytes and effect of inflammation factors on it
- VernacularTitle:原代培养的小鼠星形胶质细胞CD46、CD55、CD59的表达及炎症刺激因子对其的影响
- Author:
Xuedan CHEN
;
Yun BAI
;
Jiaxiang XIONG
;
Min SONG
- Publication Type:Journal Article
- Keywords:
CD46;
CD55;
CD59;
primary murine astrocytes
- From:Journal of Third Military Medical University
2003;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify the expression of complement regulatory protein CD46, CD55, and CD59 on primary murine pallium astrocytes and the effect of inflammatory factors on it in order to lay the foundation for studying the complement system in AD. Methods The primary murine astrocytes were cultured and purified. The expression of CD46, CD55, and CD59 on the levels of mRNA and protein was assayed by immunofluorescence before and after the stimulation of LPS and IFN-?. Results The expression of CD59 mRNA was confirmed, but the expression of CD46 and CD55 was indefinite. There was no significant difference between stimulation and non-stimulation groups. Immunofluorescence results indicated that CD59 was positive, while CD46 and CD55 were weakly positive. Conclusion Protectin CD59 expresses copiously on primary murine astrocytes, which presumably protects astrocytes from the lysis of complement.
