Screening and identification of phage antibodies against human lipopolysaccharide binding protein
- VernacularTitle:人源脂多糖结合蛋白单克隆抗体的筛选及初步鉴定
- Author:
Jia ZOU
;
Xiaodong GE
;
Yousheng LIU
;
Yanli YANG
- Publication Type:Journal Article
- Keywords:
human LBP;
phage antibody library;
monoclonal antibody
- From:Journal of Third Military Medical University
2003;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To obtain and identify monoclonal antibody against human lipopolysaccharide binding protein (LBP). Methods Three clones of antibody with better activity against human LBP were isolated after human antibody library screening by human LBP and 5 rounds of enrichment. After plasmid extracting, geneⅢ cutting, self-recircling and electric transforming, soluble Fab was expressed in E.coli. XL1-blue by the induction of IPTG and its characteristics were determined. Results The positive antibody was concentrated to 8.16?104 folds and the highest activity of the three clones was 106 . Conclusion Phage antibody against human LBP has been obtained successfully, which lays a solid foundation for researching its function.
