Expression variation of kidney OATP-3 relating to rocuronium metabolism in rats during anhepatic phase
- VernacularTitle:大鼠无肝状态肾脏罗库溴铵相关转运蛋白OATP-3及其基因的表达变化的研究
- Author:
Yifei CHEN
;
Guocai TAO
;
Jian CUI
;
Bin YI
- Publication Type:Journal Article
- Keywords:
rocuronium;
oatp-3;
anhepatic phase;
gene;
Western blotting;
RT-PCR
- From:Journal of Third Military Medical University
2003;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression variation of kidney OATP-3 in rats relating to rocuronium metabolism in anhepatic phase and to primarily explain the reason of extrahepatic metabolism characters of rocuronium. Methods Twelve rats were distributed to 2 groups randomly with 6 in each: Group A (control group) and Group B in which the hepatic portal devascularization was performed for 60 min. Kidney tissues of the rats were taken. oatp-3 mRNA was detected by RT-PCR and OATP-3 protein by Western blotting. Results The expression levels of kidney OATP-3 mRNA and protein in Group B were significantly higher than those in Group A(P
